Visual Inventory of Surface N-glycoproteins (VISUN)

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Quick Stats:

>100 human cell types

>40 rodent cell types

>2600 cell surface glycoproteins

>600 experiments

The Visual Inventory of Surface N-glycoproteins (VISUN (pronounced vision)) is a compendium of experimentally verified cell surface proteins, currently >2600 proteins, housed in an SQL database in the Gundry lab.

VISUN contains data from classic CSC, μCSC and similar experimental workflows that have been applied to a wide variety of human and rodent cell lines and primary cell types, including data from published literature. VISUN continues to grow as we analyze new cell types. All MS data are processed through our standardized bioinformatics platform for consistent analyses, including cScIFTING, GlycoLA, and CSCombiner.

While a subset of data contained in VISUN is already collated in the Cell Surface Protein Atlas (http://wlab.ethz.ch/cspa/), a majority of the data contained in VISUN are not in the CSPA. Moreover, as a result of our unique bioinformatics tools, all data have been extensively annotated with ontology, functional, and glycosite information.

Analyzing data within the VISUN repository provides a rapid and unique strategy to identify proteins that are ubiquitously expressed among a wide range of cell types, as well as those that are more restricted to a specific cell type, disease status, or lineage. Although the failure to identify a protein by CSC is not conclusive evidence of the protein’s absence in a cell type, nor has every cell type yet been mapped, these comparisons are rapid first steps to narrow down preliminary targets for next level validation.